|Comparatively Few Genes and Proteins Guide Nervous System Wiring|
|SciMed - Neuroscience|
|TS-Si News Service|
|Monday, 13 February 2012 03:00|
La Jolla, CA, USA. There is an unexpected feature of early brain development that helps to explain how complex neuron wiring patterns are programmed using just a handful of critical genes.
The findings may clarify embryonic development of the nervous system, a time axons when move with specificity to their targets. Learning how motor neurons control muscle movements can lead to new therapies for neurological disorders such as amyotrophic lateral sclerosis (ALS), and provide insight into certain cancers.
Salk Institute researchers discovered that only a few proteins on the leading edge of a motor neuron's axon and within the extracellular soup it travels through guide the nerve as it emerges from the spinal cord. The axon is the neuron's outgoing electrical wire; molecules can attract or repel the axon, depending on the long and winding path it must take to finally connect with its target muscle. The work is descibed in the journal Cell.
Image courtesy Dario Bonanomi, Salk Institute for Biological Studies.
This image shows the nerves (labeled in green) that control body movements emerging from the spinal cord of a mouse (upper left, in cross section) and connecting to muscles in the base of the leg.
Salk Institute researchers discovered that a combination of genes direct nerves to split in the leg (lower right) to make the proper connections with their target muscles during early development."The budding neuron has to detect the local environment it is growing through and decide where it is, and whether to grow straight, move to the left or right, or stop," says the study's senior investigator, Sam Pfaff, a professor in Salk's Gene Expression Laboratory and a Howard Hughes Medical Institute (HMMI) investigator.
"It does this by mixing and matching just a handful of protein products to create complexes that tell a growing neuron which way to go, in the same way that a car uses the GPS signals it receives to guide it through an unfamiliar city."
The brain contains millions of times the number of neuron connections than the number of genes found in the DNA of brain cells. This is one of the first studies to try and understand how a growing neuron integrates many different pieces of information in order to navigate to its eventual target and make a functional connection.
"We focused on motor neurons that control muscle movements, but the same kind of thing is going on throughout embryonic development of the entire nervous system, during which millions of axons make trillions of decisions as they move to their targets," he says. "It is the exquisite specificity with which they grow that underlies the basic architecture and proper function of the nervous system."
These findings might eventually shed new light on a number of clinical disorders related to faulty nerve cell functioning, such as ALS, which is also known as Lou Gehrig's disease, says the first author on the paper, Dario Bonanomi, a post-doctoral researcher in Pfaff's laboratory. "These are the motor neurons that die in diseases like Lou Gehrig's disease and that are linked to a genetic disorder in children known as spinal muscle atrophy."
Bonanomi also says it is also jumping off point to try and understand the basis for defects that might arise during fetal development of the nervous system. "A better understanding of those signals might help to be able to regenerate and rewire circuits following diseases or injuries of the nervous system."
The researchers say the study also offers insights into cancer development, because a protein the researchers found to be crucial to the "push and pull" signaling system Ret- is also linked to cancer. Mutations that activate Ret are linked to a number of different kinds of tumors. The other protein receptors described in the study, known as Ephs, have also been implicated in cancer, Pfaff says.
"This study suggests that the way cells detect signals in their environment is likely a universal strategy," he says, "and we know that genes and proteins known to function primarily during embryonic development have been linked to cancer."
"Controlling neuronal growth requires very potent signaling molecules, and it makes sense they would be linked to disease," Pfaff says. "We hope our findings help further unravel these connections."
FundingThe study was funded by the National Institute of Neurological Disorders and Stroke (NINDS) and by the Howard Hughes Medical Institute (HMMI).
ParticipationCo-authors include, from the Salk Institute for Biological Studies, Onanong Chivatakar, Ge Bai, and Karen Lettieri; Houari Abdesselem and Brian A. Pierchala, from the University of Michigan School of Dentistry; and Till Marquardt, from the European Neuroscience Institute-Göttingen (ENI-G).
CitationRet Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Dario Bonanomi, Onanong Chivatakarn, Ge Bai, Houari Abdesselem, Karen Lettieri, Till Marquardt, Brian A. Pierchala, Samuel L. Pfaff. Cell 2012; 148(3): 568-582. doi:10.1016/j.cell.2012.01.024
● Ret is a coreceptor that signals motor axon attraction via GPI-anchored ephrin-As
● Ephrin-A reverse signaling is instructive for motor axon navigation in vivo
● Ret integrates GDNF and EphA signaling using a coincidence detection signaling mechanism
● Coreceptor hierarchies dictate signaling outcomes for GPI receptors in axon guidance
Growing axons encounter multiple guidance cues, but it is unclear how separate signals are resolved and integrated into coherent instructions for growth cone navigation. We report that glycosylphosphatidylinositol (GPI)-anchored ephrin-As function as “reverse” signaling receptors for motor axons when contacted by transmembrane EphAs present in the dorsal limb. Ephrin-A receptors are thought to depend on transmembrane coreceptors for transmitting signals intracellularly. We show that the receptor tyrosine kinase Ret is required for motor axon attraction mediated by ephrin-A reverse signaling. Ret also mediates GPI-anchored GFRa1 signaling in response to GDNF, a diffusible chemoattractant in the limb, indicating that Ret is a multifunctional coreceptor for guidance molecules. Axons respond synergistically to coactivation by GDNF and EphA ligands, and these cooperative interactions are gated by GFRa1 levels. Our studies uncover a hierarchical GPI-receptor signaling network that is constructed from combinatorial components and integrated through Ret using ligand coincidence detection.
|Last Updated on Monday, 13 February 2012 06:33|