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| Hooks And Bobbins: The DNA Sewing Machine |
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| Science - Biological Sciences | |||
| TS-Si News Service | |||
| Saturday, 12 July 2008 17:00 | |||
Tokyo, Japan. Scientists in Japan have made a micro-sized sewing machine to sew long threads of DNA into shape. Their work demonstrates a unique way to manipulate delicate DNA chains without breaking them. This is a serious contribution to solving an ongoing problem often confronted by researchers: tangled up DNA.
Scientists can diagnose genetic disorders such as Down's syndrome by using gene markers, or "probes", which bind to only highly similar chains of DNA. Once bound, the probe's location can be easily detected by fluorescence, and this gives information about the gene problem.
Detecting these probes is often a slow and difficult process, however, as the chains become tightly coiled. The new method presented by Kyohei Terao from Kyoto University, and colleagues from the University of Tokyo, uses micron-sized hooks controlled by lasers to catch and straighten a DNA strand with excellent precision and care.
"When a DNA molecule is manipulated and straightened by microhooks and bobbins, the gene location can be determined easily with high-spatial resolution," says Terao. The research team's findings appear in the journal Lab on a Chip, published by the Royal Society of Chemistry (RSC).
 Maneuvering DNA. Using miniaturised hooks and bobbins, single DNA strands can be maneuvered without breakage.
Image courtesy of the Royal Society of Chemistry (RSC).
The team use optical tweezers - tightly focused laser beams - to control the Z-shaped micro hook and pick up a single DNA "thread". The hook is barbed like an arrow, so the thread can't escape. When caught on the hook, the DNA can be accurately moved around by refocusing the lasers to new positions.
But just like thread in a sewing machine, a long DNA chain can be unwieldy - so the researchers built micro "bobbins" to wind the chain around. The lasers move one bobbin around another, winding the DNA thread onto a manageable spindle.
It is "an excellent idea to fabricate unique microtools that enables us to manipulate a single giant DNA molecule", says Yoshinobu Baba, who researches biologically useful microdevices at Nagoya University, Japan. The technology will also be useful for a number of other applications including DNA sequencing and molecular electronics, he adds.
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| Last Updated on Saturday, 12 July 2008 14:55 |
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